Antimicrobial Efficacy of Phyto-L, Thiosulfonate from Allium spp. Containing Supplement, against Escherichia Coli Strains from Rabbits

Colibacillosis, caused by enteropathogenic Escherichia coli (EPEC), is one of the most common diseases in rabbit farms, resulting economic losses due to mortality and decrease production. Until recently, antimicrobials were used both treat prevent disease on livestock leading possible risk antimicrobial resistance (AMR) selection multidrug-resistant (MDR) bacteria. Therefore, interest alternative control methods, such as use natural substances, has increased scientific community. The aim this study was evaluate efficacy Phyto-L (Pro Tech s.r.l.), a product containing organosulfur compounds (OSCs) propyl propane thiosulfonate (PTSO) from Allium spp., against 108 strains E. isolated rabbits with colibacillosis 19 farms. minimum inhibitory concentration (MIC) bactericidal (MBC) assessed. Bacterial suspensions charge CFU/mL, corresponding those found gut under pathologic conditions, tested different concentrations 20 0.15 μL/mL Phyto-L. For each strain, MIC above plated Tryptic Soy agar (TSA) without assess MBCs. MBC values ranged 1.25 5 μL/mL, respectively, depending strain tested. data showed an interesting antibacterial activity EPEC strains. could be effective preventing field application, especially considering that 104–105 CFU/g feces amount usually contents physiological condition.